ObjectiveTo realize the status in the implementation of three industry standards of central sterile supply department (CSSD), and provide a scientific basis for carrying out of the standards.MethodsAccording to three standards, investigation forms were designed by specialists, written survey on 365 hospitals in 9 provinces and field investigation on 15 hospitals in 3 provinces were performed,the implementation of three standards were investigated.ResultsOf 365 hospitals, the number of provincial and ministerial level, municipal level, and county level hospitals were 90,87 and 188 respectively. More than 94% of hospitals established CSSD management system and regulations,>90% of hospitals met the requirements of CSSD layout; All hospitals were equipped with pressure steam sterilizer, all levels of hospitals basically equipped with the necessary equipments and facilities; CSSD responsible officers of 94.52% (345/365) of hospitals participated in training on standards; 69.61% （252/362） of hospitals were using or developing CSSD information systems;>92% of the CSSD responsible officers considered that three standards played an important role in facilitating centralized management, and improving the quality of cleaning, disinfection and sterilization.ConclusionHospitals need to strengthen the management and training on standards of CSSD, management of loaner instruments and development of information system need to be standardized.