ObjectiveTo investigate the difference in healthcareassociated infection (HAI) status and antimicrobial resistance of Acinetobacter baumannii（A. baumannii） isolated from different wards, so as to provide evidence for effective prevention and therapy for HAI caused by A. baumannii.MethodsClinical data of 446 A. baumannii isolates from hospitalized patients between January 2012 and July 2013 were analyzed retrospectively. ResultsThe majority of 446 A. baumannii isolates were from patients admitted to intensive care unit (ICU, n=236,52.92%), followed by departments of respiratory diseases(n=55,12.33%) and neurosurgery (n=48,10.76%),and the strains were mainly isolated from sputum specimens (94.62%).Antimicrobial susceptibility testing with 13 kinds of commonly used antimicrobial agents showed that antimicrobial resistance of A. baumannii from ICU were highest, susceptibility rates were all <20%, the next were from department of neurosurgery, susceptibility rates were all<30%, while susceptibility rates of A. baumannii from department of respiratory diseases were all >50%.ConclusionThe difference in antimicrobial resistance of A. baumannii from different wards varied significantly, antimicrobial resistance of A. baumannii from ICU is most serious.