ObjectiveTo investigate the species and drug resistance of pathogens causing bloodstream infections in hospitalized patients,and provide scientific evidence for antimicrobial use and control of healthcareassociated bloodstream infection.MethodsFrom January 1 to December 31,2012, 16 428 blood specimens were performed blood culture,pathogens were isolated and performed antimicrobial susceptibility testing.ResultsOf 16 428 blood specimens from 5 546 patients, 384 (6.92%) were positive for blood culture, 398 pathogenic isolates were detected, of which grampositive bacteria, gramnegative bacteria, and fungi accounted for 23.62% (n=94),68.34% (n=272), and 8.04% (n=32) respectively, positive rate of blood culture were highest in 61-80 age group(8.26%), the top five departments of positive rate of blood culture were departments of burn, traditional Chinese medicine, cardiac intensive care unit, transplantation and traumatology; grampositive cocci were highly susceptible to vancomycin, teicoplanin and linezolid, one Enterococcus faecium strain was found to be resistant to vancomycin; Among gramnegative bacilli, Enterobacteriaceae were highly susceptible to amikacin and carbapenems; drug resistance rates of Acinetobacter baumannii and Pseudomonas aeruginosa to carbapenems was70.97% and 35.90% respectively. ConclusionGramnegative bacteria are the major pathogens causing bloodstream infection, positive rate of blood culture of elderly people is high. It is necessary to conduct regular surveillance on distribution and drug resistance of pathogens.