ObjectiveTo investigate the major genotypes and prevalence of metalloβlactamases (MBLs) in Enterobacteriaceae from a hospital in 2007-2011. MethodsAll strains of Enterobacteriaceae were isolated from clinical specimens by routine method, antimicrobial susceptibility testing was performed by disc diffusion method(KirbyBauer) and agar dilution method, MBLs was screened by both modified Hodge test and Etest  , MBLs genes were amplified by polymerase chain reaction (PCR), the transference of IMP8encoding plasmid was performed by conjugation test , the homology of  MBLsproducing strains was detected by pulsedfield gel electrophoresis (PFGE). ResultsOf 3 297 Enterobacteriaceae isolates ,41 were   resistant to carbapenems, and phenotype confirmation test showed 14 strains produced carbapenemase, 3 Klebsiella pneumoniae isolates carrying blaIMP8 belonged to the same clone, which can be transferred to others by plasmid.ConclusionThree blaIMP8carrying Klebsiella pneumoniae isolates were found in this hospital，and has a high degree of  homology.