ObjectiveTo improve cleaning methods of ground towel in operating room , standardize the cleaning of ground of operating room, prevent and control the occurrence of healthcareassociated infection (HAI) ,and improve management quality.MethodsOperating room mops were changed into detachable mops, ground towels were cleaned and disinfected by automated washing machine (mechanical cleaning group), disinfection effect and time need to finish cleaning between mechanical cleaning group and manual cleaning group were compared.ResultsThe qualified rates of disinfection effect of ground and ground towels in mechanical cleaning group were both 96.67%(29/30) , in manual cleaning group was 83.33%（25/30）and 66.67%（20/30）respectively；the time need to complete the cleaning and disinfection of operating ground and batch cleaning of towels in mechanical cleaning group was（1.91±0.37）and （35.00±2.47）minutes respectively，and in mechanical cleaning group was（4.53±1.56 ）and （41.00±5.33）minutes respectively；there was significant difference between two groups (all P<0.01). ConclusionMechanically towel cleaning method can effectively improve ground cleaning effectively and shorten cleaning time.