ObjectiveTo evaluate the effect of improved cleaning and disinfection method for subclavian venipuncture dressing change to prevent catheterassociated infection. Methods120 hospitalized patients with right subclavian venipuncture at an intensive care unit in JanuaryJune 2012 were divided into control group (n=60) and  experimental  group(n=60) according to venipuncture date. Control group adopted conventional dressing change : disinfected skin at and around puncture point by 2% iodine tincture, then used 75% alcohol for deiodination ; experimental  group adopted improved dressing change : Wiped skin at and around puncture point three times by normal saline, cleaned catheter, disinfected skin around puncture point (avoid puncture point) three times by 75% alcohol,  disinfected skin at and around puncture point three times by 0.5% iodine tincture , then disinfected catheter . Associated infection rate between two groups were compared . ResultsFocal infection rate and CRBI rate of experimental group were both lower than control group(5.00% vs 16.67% ;1.67% vs 13.33%), the difference was statistically different (P< 0.05). ConclusionEffect of improved dressing method is better than the conventional dressing method, it can effectively prevent occurrence of focal infection and CRBI.