ObjectiveTo realize types and changing trends of multidrug resistant organism(MDROs) infection, so as to provide reference for preventing and controlling  MDRO healthcareassociated  infection (HAI) . MethodsBacteria and MDROs isolated from patients in a general hospital from October 1, 2010 to September 30,2013 were analyzed. ResultsA total of 5 223 bacteria strains were isolated, 1 213 (23.22%) of which were MDROs. χ2 trend test showed that the percentage of MDROs increased year by year (χ2=16.885, P<0.001).Of all MDROs, rate of HAI strains ,communityassociated infection (CAI) strains, colonized strains ,and contamination strains were 37.51%(n=455), 50.54%(n=613) , 9.23%(n=112) , and 2.72%(n=33) respectively . From 2010 to 2013, the percentage of MDRO HAI showed decreased trend (χ2=13.477,P<0.001), CAI and colonization showed increased  trend (χ2=4.536, P=0.033) ,the main infection site was respiratory tract(84.49%，n=997) ,followed by urinary tract , skin and soft tissue, and bloodstream.  ConclusionMDRO infection become more and more serious,management of patients with MDRO CAI  and colonization  has become the focus of HAI prevention and control.