ObjectiveTo realize the incidence of healthcareassociated infection (HAI),the rate of invasive procedure and related infection rate, use of antimicrobial agents and distribution of pathogens in gereral intensive care units (GICUs) of hospitals in China. MethodsHAI crosssectional investigation data  of GICUs reported to China HAI Surveillance Network were summarized and analyzed.ResultsOf 1 313 hospitals, 621（47.30%）had GICUs；a total of 5 887 patients were surveyed, 1 634 patients developed 1 962 times of infection，HAI prevalence rate and case rate was 27.76% and 33.33% respectively. The main infection sites were lower respiratory tract（70.39%），urinary tract （12.79%） and blood（2.86%）.The application rate of urinary catheter, arteriovenous catheter and ventilator was 53.52%（n=3 151）,37.05%（n=2 181）and 35.62%（n=2 097）respectively，and related prevalence of urinary tract infection, pneumonia and blood stream infection was 4.67%,20.41%, and 0.60%，respectively，which accounting for 58.57%,30.99%, and 23.21% of urinary tract infection, pneumonia and blood stream infection respectively, the overall prevalence of above invasive procedurerelated infection accounted for 29.97%（588/1 962）of the overall HAI prevalence.A total of 1 795 isolated pathogens causing HAI were detected，the top three were Pseudomonas aeruginosa（20.78%）, Acinetobacter baumannii (17.99%) and Klebsiella pneumoniae (11.64%). The usage rate of antimicrobial agents was 71.58%（n=4 214），rate of specimens delivered for detection  was up to 75.27%（2 553/3 392）.ConclusionPrevalence and antimicrobial use in patients in GICUs are all high, all kinds of invasive procedure are frequent, 30% of HAI are related to invasive procedure; infection caused by nonfermentative bacteria is serious, pathogen detection is well performed.