ObjectiveTo explore the risk factors for methicillinresistant Staphylococcus aureus (MRSA) infection of refractory wound, and provide reference for clinical prevention and control.  MethodsClinical data of patients who were isolated Staphylococcus aureus (SA) from wound at the burn ward in a hospital from January 2006 to December 2013 were analyzed, patients were divided into four groups according to whether the isolated SA were MRSA and whether  SA  were from refractory wound or from nonrefractory wound. Risk factors for MRSA infection of refractory wound were analyzed.ResultsA total of 112 isolates of SA were isolated from wound, statistical differences existed in the length of hospital stay, recent invasive operation, and recent antimicrobial use between patients in refractory wound MRSA group and refractory wound methicillinsensitive SA (MSSA) group (all P<0.05); nonconditional logistic regression analysis revealed that length of hospital stay>30 days (OR 95% CI：1.14-30.69) and recent invasive operation (OR 95% CI：1.41-17.84)were independent risk factors for refractory wound MRSA infection. There were statistically differences in previous MRSA infection, burn depth, recent operation and recent antimicrobial use between refractory wound MRSA group and nonrefractory wound MRSA group(all P<0.05) ; nonconditional logistic regression analysis revealed that recent antimicrobial use (OR 95% CI：2.080-26.800） was independent risk factor for the persistence of MRSA infection  of refractory wound. ConclusionShortening the length of hospital stay, reducing invasive operation, and using antimicrobial agents rationally are helpful for the prevention and control of MRSA infection of refractory wound.