ObjectiveTo investigate colonization status and risk factors of multidrugresistant organisms(MDROs) in a surgical intensive care unit (SICU),and provide a basis for active clinical screening of MDROs.  MethodsFrom June 1,2013 to August 31,2013, patients who admitted to SICU≥24 hours were performed active screening, the colnization status of methicillinresistant Staphylococcus aureus (MRSA) and extendedspectrum βlactamaseproducing Escherichia coli/Klebsiella pneumoniae (ESBLE.coli/Kp) among patients were detected, related risk factors were analyzed. ResultsWhen patients who admitted to SICU≤48 hours, the detection rate of MRSA and ESBLE.coli/Kp was 11.00％ and 73.00％ respectively；when admitted to SICU > 7 days,the increased detection rate of MRSA and ESBLE.coli/Kp was 16.67% and 44.44% respectively. Patients stayed in hospital >7 days before admitting to SICU (OR95%CI：4.48［1.21-16.65］) was  an independent risk factor of carrying MRSA when admitting to SICU, APACHE Ⅱ score ≥16 (OR95%CI：6.36［1.47-27.54］) was an independent risk factor of carrying MRSA 48 hours after admitting to SICU. ConclusionWhen patients admitted to SICU, the carrying rate of MDROs is high, isolation rate rises with prolonged length of SICU stay. Hospitals should carry out MDRO colonization screening project among patients and implement effective isolation control measures to reduce the incidence of healthcareassociated infection.