ObjectiveTo investigate the distribution and antimicrobial resistance of pathogens causing diabetic foot ulcer(DFU).  MethodsSecretion specimens of DFU in patients at several hospitals in Qingdao were collected and performed bacterial culture and antimicrobial susceptibility testing.  ResultsA total of 216 pathogens were obtained from 137 patients with DFU. Isolation rate of gramnegative bacilli, grampositive cocci and fungi was 58.80% (n=127), 39.81% (n=86) and 1.39%(n=3) respectively. 76 （55.47%）patients were infected with single pathogen, 61 （44.53%）were infected with mixed pathogens；the top five detected pathogens were Pseudomonas aeruginosa（n=37）, Staphylococcus aureus (n=35), Escherichia coli (n=26), Staphylococcus epidermidis(n=24), and Acinetobacter baumannii(n=22). The resistance of Enterobacteriaceae to amikacin, amoxicillin / clavulanic acid, cefoxitin, imipenem, piperacillin / tazobactam, ticarcillin / clavulanic acid were 0-16.67%；the resistance of Pseudomonas aeruginosa to  imipenem, tobramycin, amikacin, cefepime, gentamicin, and quinolones were 2.70%-18.92%；the resistance of Acinetobacter baumannii to imipenem and levofloxacin were low，but to the other antimicrobial agents were all above 30%. ConclusionThe major pathogens from DFU were gramnegative bacilli, the next were grampositive cocci, antimicrobial resistance patterns of each species are quite different, clinicians should choose antimicrobial agents according to antimicrobial susceptibility testing results.