ObjectiveTo analyze strains of Proteus mirabilis (P.mirabilis) and Proteus vulgaris (P. vulgaris) isolated in a hospital, detect resistance to commonly used antimicrobial agents,and provide reference for rational application of antimicrobial agents in clinic. Methods172 P.mirabilis isolates and 68 P. vulgaris isolates isolated between January 1, 2011 and June 30,2013 were analyzed,  antimicrobial  resistance susceptibility testing were performed by disk diffusion method,  data were analyzed with WHONET 5.4 software. ResultsP. mirabilis strains were mainly isolated from wound secretion（26.74%）,sputum（22.68%）and urine（18.61%）, P. vulgaris were mainly from wound secretion（48.53%）,urine（17.65%）and sputum（11.77%）. The resistance rates of P. mirabilis to ampicillin and trimethoprim/sulfamethoxazole were both>45.00%； the resistance rates of P. vulgaris to cefazolin and trimethoprim/sulfamethoxazole was 86.76% and 41.18% respectively；the resistance rates of P.mirabilis and  P. vulgaris to piperacillin/tazobactam, cefotaxime，ceftazidime, cefepime, carbapenems (ertapenem and meropenem) and amikacin were all <20.00%. ConclusionThe resistance rates of P.mirabilis and  P. vulgaris  to piperacillin/tazobactam, cefotaxime, ceftazidime, cefepime, ertapenem, meropenem and amikacin are all high, and can be used as the empirical medication for the treatment of related infection.