ObjectiveTo investigate antimicrobial resistance and change trends of Ureaplasma urealyticum (Uu) from reproductive system of female patients at a traditional Chinese medicine hospital in three consecutive years. MethodsAntimicrobial resistance of Uu isolated from gynaecology leucorrhea and cervical secretion specimens of patients of dermatology &  sexually transmitted diseases department and gynaecology department from January 2011 to December 2013 were analyzed retrospectively. ResultsA total of 327 Uu strains were isolated, except resistance rates of Uu to doxycycline（5.23%-6.12%） and  josamycin（0-1.96%） remained at a relatively low level in three consecutive years,  resistance rate  to the other  antimicrobials showed an upward trend year by year,but the difference was not significant (P＞0.05）.The resistance of Uu to ofloxacin, clarithromycin and sparfloxacin were relatively high（42.11%-61.40%）, to josamycin, doxycycline and minocycline were lower（0-13.07%）, and multidrug resistance was serious. ConclusionAntimicrobial resistanc of Uu isolated from reproductive system of female patients in this hospital is high. It is necessary to realize the status and change trends of antimicrobial resistance of Uu for the treatment of disease and control of the emergence of multidrug resistance pathogens.