ObjectiveTo study the effect of disinfectant resistance gene qacEΔ1 on the drug resistance of commonly isolated gramnegative bacteria.MethodsGramnegative bacteria isolated from neonates in a neonatal intensive care unit(NICU) in JulyDecember 2013 were detected and performed antimicrobial susceptibility testing,qacEΔ1 gene were detected by polymerase chain reaction. ResultsA total of 608 gramnegative bacteria were collected, the major isolates were Klebsiella pneumoniae (n=216), followed by Escherichia coli (n=128), Enterobacter cloacae (n=77), Acinetobacter baumannii (n=62),and Pseudomonas aeruginosa (n=19). The positive rate of  qacEΔ1 gene in above five bacteria was 62.75%(315/502), Acinetobacter baumannii was the highest (87.10%). qacEΔ1positive  and negative strains showed statistical difference in the resistance rates to piperacillin,  cefepime, aztreonam, imipenem, and compound sulfamethoxazole （all P≤0.05）.The resistance rate of five strains to piperacillin, ceftazidime, cefepime, and aztreonam  were all high.ConclusionThe positive rates of qacEΔ1 in five major gramnegative bacteria from neonates in  NICU are all high, qacEΔ1positive strains have even higher resistance rates to piperacillin, cefepime, aztreonam, imipenem, and compound sulfamethoxazole.