Objective To explore the effect of implementation of prevention and control measures on reducing the incidence of multidrugresistant organisms(MDROs) healthcareassociated infection(HAI) rate. MethodsImplementation of prevention and control measures on MDROs were strengthened in a hospital in 2012, the relationship between implementation status and change in MDROs infection in the whole hospital were analyzed statistically.ResultsThrough continuous monitoring and tracking, the implementation of prevention and control measures on MDROs continued to be improved, MDRO HAI rate of the second half year was significantly lower than the first half year (［0.29%，119/41 579］ vs［0.49%，186/38 252］）（χ2=20.95，P＜0.01）. ConclusionContinuous monitoring and tracking of MDROs is helpful for implementing the prevention and control measures on MDROs infection, as well as reducing the incidence of HAI caused by MDROs .