ObjectiveTo explore the effect of comprehensive intervention measure on antimicrobial use and incidence of healthcareassociated infection(HAI)  in patients in  sports medicine department. MethodsAntimicrobial use and incidence of HAI in hospitalized patients in the surgical departments (sports medicine department and other surgical departments) of a hospital between January 2006 and  December 2010 were analyzed retrospectively(before intervention),from January 2011, comprehensive intervention based on clinicalcentered multidepartment cooperation was adopted by sports medicine department, the other surgical departments over the same period were not comprehensively intervened; antimicrobial use and incidence of HAI in patients in surgical departments between January 2012 and  December 2013 were monitored prospectively (after intervention ), effectiveness of intervention was evaluated. ResultsAntimicrobial use and incidence of HAI in patients in sports medicine department after intervention were both lower than before intervention ( 55.75% vs  95.26%, χ2 =753.42;  0.10% vs 0.32% , χ2 =15.13, both P＜0.01). Antimicrobial use in patients in other surgical departments after intervention was lower than that before intervention（65.63% vs 73.79% , χ2=251.57, P＜0.01）; incidence of HAI was higher than that before intervention（0.55% vs 0.42% , χ2=19.04, P＜0.01）.  ConclusionComprehensive intervention measure based on clinicalcentered multidepartment cooperation is safe and  effective, it can reduce antimicrobial use and incidence of HAI  in patients in sports medicine department.