ObjectiveTo explore the effect of Pareto analysis in  management on hospital cleaners in infectious disease specialty hospital.MethodsThe implementation of healthcareassociated infection(HAI) prevention and control measures among 32 cleaners were supervised, defects items were analyzed by Pareto analysis,  top priority problems which needed to be dealt with were found out,  the quality was improved by strengthening the training, formulating and perfecting standard operation procedures, providing clear signs,  and establishing competencebased strategic management.  ResultsBefore rectification, there were 121 cases of incorrect implementation, the main defects items were “implementation of standard precautions”， “disinfection and isolation procedures in each location”, “preparation of  disinfectant”, and “hand hygiene ”,  cumulative percentage  of  these four items was 76.03%（92 cases）. Pareto analysis and expert brainstorm method showed that the main causes of incomplete implementation of HAI prevention and control measures among cleaners were lack of HAI knowledge，incomplete operating process and all kinds of signs，varied attention on HAI management among administrators，and less stringent supervision. After rectification，incorrect implementation dropped to 48 cases，cumulative percentage of above four measures was 72.92%（35 cases）.The implementation rate of HAI prevention and control measures among 32 cleaners were statistically different before and after rectification (P＜0.05）.ConclusionPareto analysis can quickly find out the key problems which need to be solved, improve the efficiency of HAI prevention and control management, and has important significance to reduce the occurrence of HAI.