ObjectiveTo explore the distribution and antimicrobial resistance of pathogens causing urinary tract infection(UTI)  in patients with prostatic hyperplasia， and provide reference for clinical diagnosis and  rational antimicrobial therapy.MethodsUrine culture results of 171 patients with prostatic hyperplasia complicated with UTI from February 8, 2012 to October 12, 2013 were analyzed retrospectively.ResultsA total of 192 bacterial isolates were isolated from 171 patients, gramnegative bacteria accounted for 79.17%, 37.50% of which were Escherichia coli (E. coli) strains, and 18.75% were Klebsiella pneumoniae (K. pneumoniae); grampositive bacteria and Candida accounted for 18.23% and 2.60% respectively. Of E. coli and K. pneumoniae, the proportion of  extendedspectrum βlactamase (ESBLs) strains were relatively higher, which was 72.77% and 63.89% respectively, resistant rates of ESBLsproducing E. coli to piperacillin. cefuroixme， ceftazidime, ceftriaxone, aztreonam, and levofloxacin were all  significantly higher than nonESBLs strains, resistant rates of ESBLsproducing K. pneumoniae to piperacillin, cefoxitin, aztreonam, levofloxacin，and compound sulfamethoxazole were all  significantly higher than nonESBLs strains. Resistant rates of Pseudomonas aeruginosa to imipenem and meropenem were both 50.00%, susceptibility rates of Enterococcus to penicillins and aminoglycosides were both low, linezolid and vancomycinresistant strain was not found.ConclusionOrganisms isolated from patients with UTI associated with prostatic hyperplasia have high antimicrobial resistance, clinical therapy should be based on characteristics of bacterial distribution and antimicrobial resistance