ObjectiveTo improve hand hygiene compliance among healthcare workers (HCWs) in a stomatological hospital， and effectively reduce healthcareassociated  infection(HAI) rate.MethodsAccording to WHO hand hygiene guideline and implementation scheme of hand hygiene, a systemic and step by step hand hygiene promotion plan was formulated, the plan was divided into five steps: full alert, baseline investigation, improvement, tracking, and retrospective analysis. Hand hygiene compliance among HCWs before and after the promotion of activities was analyzed and compared.ResultsThrough hand hygiene promotion activities, the questionnaires showed that average score of knowledge about hand hygiene among HCWs enhanced from 58.33  to 77.40 (increased by 32.69%); the average hand hygiene compliance rate increased from 24.28%（59/243）to 49.81%（133/267），the difference was significant（χ2=35.331，P =0.000）；hand hygiene status at five moments for hand hygiene improved markedly.ConclusionHand hygiene promotion has a significant influence in hand hygiene among HCWs in this stomatological hospital.