ObjectiveTo investigate the carriage of phenol soluble modulinα (PSMα) in methicillinresistant Staphylococcus aureus（MRSA）and influence in human peripheral blood neutrophils， so as to provide theoretical basis for guiding clinical treatment and identifying pathogenesis of MRSA.MethodsNinety MRSA isolates which were isolated from clinical specimens and performed SCCmec genotyping were collected， PSMα gene was amplified by polymerase chain reaction（PCR), and the effects of PSMα gene on  hunman peripheral blood neutrophils was studied by cell morphology analysis.ResultsOf 86 hospitalacquired MRSA(HAMRSA) isolates，78 were positive for PSMα gene，the positive gene was 90.70%; Of 4 communityacquired MRSA(CAMRSA)， 2 were positive for PSMα gene，the positive rate was 50.00%; neutrophil death rate in PSMα positive group was significantly higher than normal saline group, PSMα negative group and ATCC 25923 group, but there was no significant difference between  PSMα negative group and ATCC 25923 group.ConclusionHAMRSA detected in this region carry PSMα gene, and PSMα secreted by PSMα positive MRSA has the activity of promoting the lysis of neutrophils.