ObjectiveTo realize drug resistance pattern and disinfectantresistant genes  (qacA/B)  of Staphylococcus aureus (S. aureus) from neonates at a neonatal ward.MethodsS. aureus from neonates between January and December 2013 were identified and performed antimicrobial susceptibility testing by VITEK 2 Compact system, qacA/B gene was detected by polymerase chain reaction (PCR). Results225 S. aureus strains were isolated,which were mainly from respiratory tract (72.44%) and umbilical  secretion (14.33%); isolation rate of methicillinresistant S.aureus (MRSA) was 8.89%(n=20); antimicrobial resistant rate of MRSA was generally higher than methicillinsensitive S.aureus (MSSA)；levofloxacin, moxifloxacin, tigecycline, vancomycin, linezolid， and nitrofurantoin were all had high antimicrobial activity against all S.aureus， susceptibility rates were all 100%. 21( 9.33%) S.aureus carried qacA/B, 3(15.00%) of which were MRSA, and 18(8.78%) were MSSA.ConclusionDrug resistance of MRSA isolated from neonates in neonatal ward is serious, MRSA strains carry qacA/B, which should be paid more attention.