ObjectiveTo evaluate the feasible hand hygiene intervention methods to improve health care workers’ (HCWs) hand hygiene compliance, and reduce healthcareassociated infection(HAI) rate.MethodsThe surgical department in a hospital was selected as intervention department, intervention department was conducted baseline investigation in JanuaryFebruary 2013, multimodel intervention measures were conducted  between  March  2013 and April 2014, postintervention survey was performed by the same method as baseline survey in MayJune 2014, hand hygiene of HCWs as well as  occurrence of HAI between before  and after intervention was compared respectively. ResultsAfter intervention, HCWs’ hand hygiene compliance  before and after patient contact both enhanced significantly; HCWs’ hand hygiene compliance rate increased from 53.32% before intervention to 69.27% after intervention, the difference was significant(χ2=26.19, P<0.001). HCWs’ hand hygiene modes before patient contact: liquid soap plus running water before intervention(67.02%), alcoholbased hand rub after intervention (61.08%); HCWs’ hand hygiene modes after patient contact : both liquid soap plus running water before and after intervention (86.40% and 70.41% respectively). Before intervention， hand dry method of  HCWs before and after patient contact was by wiping with uniforms， after intervention was by wiping with paper towel. HAI in intervention department decreased from 3.08% before intervention to 2.00% after intervention, the difference was significant(χ2=7.76, P=0.005).ConclusionMultimodel intervention measures in intervention department can effectively improve HCWs’ hand hygiene compliance rate.