ObjectiveTo investigate the incidence of healthcareassociated infection(HAI) in hospitalized patients ≥65 years at a hospital in Hunan  Province.MethodsData of patients with HAI in this hospital in 2011—2013 were classified and analyzed statistically.ResultsA total of 47 626 patients were investigated, 1 068 cases  of HAI occurred, all cases were single site infection， incidence and case rate of HAI were both  2.24%, incidence in patients aged <65 years  (265 years old) was 0.98% （234/23 998），and ≥65 years  was 3.53%（834/23 628）, difference was significant between two groups (χ2=354.44, P<0.001). HAI rate in patients aged ＜65, 65~, 70~, 75~, 80~, 85~, and ≥90 years was 0.98%, 1.59%, 1.28%, 2.77%, 5.20%, 6.93%, and 9.43% respectively.  The major infection site was lower respiratory tract (59.95%, n=500), the main detected pathogens were Pseudomonas aeruginosa (19.43%) and Escherichia coil(18.72%).ConclusionThe incidence of HAI in the elderly patients increased with age increasing, the main infection site is lower respiratory tract.