ObjectiveTo evaluate the effect of evidencebased bundle intervention strategy on reducing the incidence of central lineassociated bloodstream infection (CLABSI).MethodsProspective and multicenter study was adopted, patients admitted to 54 intensive care units(ICUs) of 41 hospitals and with central venous catheters(CVCs) between October 1, 2013 and September 30,2014 were monitored . Baseline data between October 2013 and March 2014 were collected as preintervention data; from April to September 2014, the participated hospitals performed intervention strategy, postintervention data were compared with preintervention data.ResultsThe usage rate of CVCs before and after intervention was significantly different (44.18% vs 44.63%,χ2=5.526, P=0.019). Incidence of CLABSI before and after intervention was not significantly different(RR ，0.82［ 95%CI, 0.59-1.13］，P=0.10). Constituent ratio of catheter insertion sites between pre and postintervention was significantly different (χ2=76.264, P<0.001), femoral vein catheterization rate as well as proportion of two and above catheter insertion sites after intervention decreased（17.25% VS 13.72%; 2.27% VS 1.44%,respectively）; hand hygiene implementation rate and accuracy rate after intervention were both higher than before intervention (79.73% vs 76.14%, P<0.001; 91.47% vs 74.26%, P<0.001,  respectively); constituent ratio of skin disinfectant application before and after intervention was significantly different(χ2=3.861, P<0.001),  proportion of chlorhexidine ethanol increased (29.62% VS 50.56%); except daily assessment and record, compliance to other prevention and control measures before and after intervention were all significantly different(all P<0.001); utilization rate of maximal sterile barrier, qualified rate of dressing of operators,and port disinfection were all significantly enhanced.ConclusionBundle intervention in intubation and maintenance are implemented effectively, but intervention effect on CLABSI needs further study.