ObjectiveTo investigate current status of healthcareassociated  infection(HAI) and communityassociated infection(CAI) of different levels of hospitals in Xinjiang.MethodsInfection, antimicrobial use and pathogen detection in patients in secondclass   and above hospitals in Xinjiang on March 20,2014 were performed crosssectional survey by  bedside visiting  and medical record reviewing methods.ResultsA total of 66 208 patients in 132 hospitals were investigated, HAI prevalence rate and case prevalence rate was 1.83% and 2.02% respectively;  CAI prevalence rate and case prevalence rate was 24.53% and 24.83% respectively. HAI prevalence rate in thirdclass hospitals was higher than secondclass hospitals (2.58% vs 1.44%, P＜0.001）,while CAI prevalence rate was lower than secondclass  hospitals (17.84% vs 28.01%, P＜0.001）.HAI prevalence rate was highest in intensive care unit (14.91%),  CAI was  highest in pediatric department. The common infection sites were respiratory tract, surgical site and urinary tract. Antimicrobial usage rate was 31.27%，antimicrobial usage rate in secondclass hospitals was higher than that of the thirdclass hospitals(［35.95%, n=15 642］ vs ［22.32%,n=5 064］,χ2=1 288.974,P＜0.001),pathogen detection rate  in thirdclass hospitals was higher than that of the secondclass hospitals(［59.40%, n=2 224］ vs ［53.59%,n=6 436］,χ2=38.964,P＜0.001) Gramnegative bacilli were main isolated pathogens, the top 3 pathogens were Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii.ConclusionThe crosssectional survey can reflect infection status of different levels of  hospitals, surveillance of targeted microbial detection and rational antimicrobial use should be strengthened, management of infection in high risk population and key sites should be paid close attention.