ObjectiveTo analyze the distribution and antimicrobial resistance of pathogenic strains in wound infection，and provide scientific evidence for rational use of antimicrobial agents in  treatment of wound infection as well as  reducing the emergence of drugresistant organisms.MethodsData about pathogenic strains isolated from wound specimens of patients with wound infection in a hospital between June 2011 and April 2014 were analyzed retrospectively.  ResultsA total of 965 pathogenic strains were isolated from wound specimens，the main infection sites were limbs（50.47%）；infected  patients mainly distributed in department of orthopaedic surgery（44.97%）；trauma  and incisional wound infection were the major infection types(47.98%, 36.48%, respectively). Among isolated pathogens, grampositive bacteria， gramnegative bacteria, and fungi accounted for 37.20%, 59.59%, and 3.21% respectively. The main grampositive bacteria were highly susceptible to vancomycin, teicoplanin，and linezolid，the main gramnegative bacteria were highly resistant to most commonly used antimicrobial agents except cefoperazone / sulbactam, piperacillin / tazobactam， imipenem, and meropenem.ConclusionPatients with wound infection are mainly distributed in department of orthopedics，  the main infection types are trauma and surgical site infection,  antimicrobial resistant rates of the major grampositive and gramnegative bacteria are both high. Surgeons, especially orthopedics surgeons, should pay attention to the culture of pathogens and monitoring of antimicrobial susceptibility, use antimicrobial agents rationally，and strengthen the prevention and control of surgical site infection，so as to reduce the infection incidence and occurrence of drugresistant organisms.