ObjectiveTo evaluate the effect of  tourniquet on incisional  infection  based on animal model, and evaluate the effect of ischemia preconditioning on incisional  infection, so as to provide experimental basis for the rational use of tourniquet in clinic.Methods100 BALB/c mice were randomly divided into 4 groups, with 25 mice in each group. Group A: tourniquet+methicillinresistant Staphylococcus aureus(MRSA) incisional infection; group B: ischemic preconditioning+MRSA incisional infection ; group C : MRSA incisional infection; group D: blank control. The survival rates, temperature, routine blood testing, and change in soft tissue beside femoral incision were compared among 4 groups.ResultsDuring observation period ,the survival rate in group A was 68%, in group B,C, and D were all 100%,  group A was lower than group B,C, and D (P＜0.05). The temperature at each time point was significantly different among group A, B, and C (P＜0.01); temperature curves of 4 groups were significantly different (P＜0.01); except basal body temperature, temperature at the other  time points among 4 groups were significantly  different(all P＜0.01); routine blood testing results (white blood cell count, total granulocyte count, and total lymphocyte count) among 4 groups  on the third, and seventh day were significantly different(all P＜0.05); on the seventh  day, routine blood testing results of 4 groups all returned to normal level, but group A was slightly higher than group B,C, and D. Section of epithelial tissue revealed that epithelial tissue in group A was not healed，inflammatory response of peripheral tissue was most serious;epithelial tissue in 10 mice in group B was healed，partial peripheral tissue revealed inflammatory response;epithelial tissue in 4 mice in group C was healed，most peripheral tissue revealed inflammatory response.ConclusionMice incisional infection will be aggravated by using lower limb tourniquet, incision healing and body function recovery time will be delayed, even lead to death. Ischemic preconditioning before making an incision model can reduce the severity of incisional infection, and shorten wound healing time.