ObjectiveTo understand the constituent and antimicrobial  resistance of pathogens causing intraabdominal infection,  so as to provide reference for clinical treatment of intraabdominal infection.MethodsPathogens causing intraabdominal infection in patients in a hospital from January 2011 to December 2013 were identified and performed antimicrobial susceptibility testing, and results were  analyzed with WHONET5.6 software.ResultsA total of 810 nonrepeated strains were isolated from 15 946 intraabdominal specimens, isolation rate was 5.08%; isolation rates of gramnegative bacilli, grampositive bacteria, and fungi were  59.88%(n=485), 33.95% (n=275), and 6.17%(n=50) respectively;  the top five pathogens were Escherichia coli (E. coli,24.20%), Enterococcus faecium (E. faecium, 15.06%), Acinetobacter baumannii (A. baumannii, 8.89%), Klebsiella pneumoniae (K. pneumoniae, 7.66%), and coagulase negative staphylococcus (CNS, 6.91%). The detection rates of extendedspectrum β1actamases(ESBLs) in E. coli and K. pneumoniae were 59.18％ and 32.79％ respectively. Enterobacteriaceae were still highly sensitive to imipenem, but carbapenemresistant Enterobacteriaceae strains accounted for 4.08%-6.67% ; multidrugresistant A. baumannii accounted for  52.11%（37/71）, methicillinresistant strains in Staphylococcus aureus and CNS accounted for 53.57％（15/28） and 71.43％（40/56） respectively, resistance rate of E. faecium to vancomycin was 8.26%.ConclusionThe main pathogens causing intraabdominal infection in this hospital are gramnegative bacilli, especially E. coli, while E. faecium is the most common grampositive pathogen, antimicrobial resistance of bacteria is still serious.