ObjectiveTo realize the isolation of multidrugresistant organisms (MDROs) from ward environment, and provide evidence for clinical prevention and control of MDRO transmission.MethodsPatients with MDRO infection in a stomatology hospital from September 2012 to July 2014 were as trial group, 12 randomly selected patients without MDRO infection were as control group, environmental object surface in patients’ ward were taken specimens and performed culture, isolated organisms were identified and performed antimicrobial susceptibility testing.ResultsOf 44 patients with MDRO infection, 13 patients’ surrounding environment were detected MDROs, the total detection rate was 29.55%（13/44）. The detection rate of methicillinresistant Staphylococcus aureus(MRSA)， multidrugresistant Acinetobacter baumannii(MDRAB)，and methicillinresistant Staphylococcus epidermidis(MRSE) were 45.45%（5/11）， 66.67% （2/3）and 66.67%（6/9） respectively. Detection rates of MRSA from nasal cavity and hands of MRSAinfected patients were 72.73% and 54.54% respectively， from nasal cavity and hands of patients’ caregivers were 36.36% and 18.18% respectively. The difference in bacterial count between trial group and control group was not significantly different (all P＞0.05).ConclusionIsolation rate of MDROs from ward environment of MDROinfected patients is higher than that of nonMDROinfected patients, monitoring, cleaning and disinfection for MDROinfected patients’ surrounding environment should be strengthened, so as to prevent the spread of MDROs in hospital.