ObjectiveTo investigate medical students’ demand for knowledge about healthcareassociated infection(HAI), explore the opportunity and method of setting up curriculum about nosocomiology.Methods447 medical students who were about to start clinical practice were investigated through questionnaires.Results434  (97.09%) questionnaires were available. 152 (35.02%) medical students had access to knowledge about HAI, 148 (34.10%) knew or learnt sixstep handwashing method, 80 students understood sixstep handwashing method through media and society (n=65, 81.25%) or school curriculum (n=15, 18.75%). 95.62% of students hoped to set up curriculum about nosocomiology, 354 (81.57%) students preferred this discipline to be set up before clinical probation; 55.07% of students hoped this course should be set up as a practice course, 44.93% thought that theory and practice should be combined. Occupational precaution measures as well as methods of disinfection and isolation were the most interesting knowledge among them.ConclusionNosocomiology should be an obligatory course in medical colleges, the semester before clinical practice is the best time for this course, the curriculum should combine theory with practice.