ObjectiveTo evaluate efficacy of routine cleaning and disinfection methods for incubators, and put forward a feasible  improvement solution. Methods30 incubators used in a neonatal intensive care unit of a hospital between December 2013 and June 2014 were chosen and randomly divided into baseline, control, and trial groups(10 incubators in each group). Baseline group and control group were disinfected by routing disinfection method (wiping internal and external surfaces of incubators with water and chlorinecontaining disinfectant), trial group adopted intensified disinfection method (wiping internal surfaces of incubators with alcohol) on the basis of routine disinfection, disinfectant efficacy of three groups were compared. ResultsIn baseline group, unqualified incubators were initially detected on the fourth day of monitoring, all incubators were contaminated in varying degrees on the seventh day of monitoring, the detection rate of unqualified specimens was 31.43% (88/280). The median  time for the initial detection of unqualified incubators in control group  and trial group were on the fifth day and seventh day respectively, there was significant difference between two groups（χ2=12.38，P＜0.05）; The unqualified rate of trial group was significantly lower than control group (15.36%［43/280］ vs 32.86%［92/280］，χ2=23.43，P＜0.05）. ConclusionIntensified disinfection with alcohol on the basis of routine disinfection method can effectively improve the disinfectant efficacy of the surface of incubators, it is convenient, inexpensive and safe, and worth to be popularized in primary hospitals.