ObjectiveTo analyze characteristics of pathogenic microbiology and antimicrobial susceptibility of Vibrio vulnificus (V.vulnificus) causing infection in patients at a hospital in Shantou City.MethodsFour patients with V.vulnificus infection admitted to a hospital between June 2013 and June 2014  were analyzed  retrospectively,  blood specimens of patients were collected for culture, blister fluid  and wound secretion were taken and inoculated on blood agar plates,  MacConkey agar plates, and thiosulfate citrate bile salts sucrose (TCBS) plates, isolated strains were  identified and performed antimicrobial susceptibility testing.ResultsAmong 4 patients with V.vulnificus infection, 3 died, 1 was cured; patients developed primary septicemia and lower extremity infection because of eating pickled crab，steamed scallop， or  contacting with seafood products after trauma. Blister fluid  from patients A and B,  wound secretion from patient C, and blood from patient D were all isolated  oxidasepositive gramnegative bacteria  which produced green colonies on TCBS plate, these strains were identified as V.vulnificus, which were resistant or intermediate to cefazolin, intermediate to cefotetan, and 1 strain was resistant to cefepime. ConclusionFour patients infected with V.vulnificus have high mortality rate （3/4）, positive rates of blister fluid  and wound secretion are higher than blood;  timely medical consultation, early antimicrobial therapy, and active debridement will help to cure the disease.