ObjectiveTo understand environmental quality status of clean operating rooms in municipal hospitals of Xi’an, and provide reference for ensuring the clean quality of operating rooms.MethodsOnsite specimen collection and detection were conducted  to detect and analyze the cleanlinss of air in clean operating rooms in 15 hospital of  Xi’an.ResultsA total of 51 operating rooms were monitored, secondary and tertiary hospitals had 24 and 27 operating rooms respectively; 26 were gradeI and 25 were gradeⅢ operating rooms. The qualified rates of  dust particle≥0.5μm / particle size in  gradeI and gradeⅢ clean operating rooms were 76.92% and 80.00% respectively, ≥5μm / particle size were 73.08% and 88.00%  respectively. The qualified rates of dust particle counts in gradeI and gradeⅢ clean operating rooms were 61.54% and 80.00% respectively，difference was not significant（χ2=2.092，P＞0.05）. The qualified rate of dust particle counts in clean operating rooms in tertiary hospitals was significantly higher than secondary hospitals(85.19% vs 58.33%, χ2=4.600，P<0.05）. The qualified rates of airborne microbes in operating zone of secondary and tertiary hospitals were 87.50% and 92.59% respectively(P>0.05), in surrounding zone were 95.83% and  92.59% respectively (both P>0.05).ConclusionDust particle and airborne microbes in some clean operating rooms in municipal hospitals of Xi’an are beyond the standard, supervision and management  should be strengthened.