ObjectiveTo explore the application value of hospital execution culture in healthcareassociated infection(HAI)  management. MethodsAll departments in a hospital from January 1, 2013 to December 31, 2014 were studied.  From January 1, 2014, ideas and fostering methods for  hospital execution culture  were applied in HAI management, the working enthusiasm, working ability, and working achievements of health care workers (HCWs) on HAI  control before and after the application of execution culture  were compared. Results53 departments in the hospital were surveyed annually in  2013 and 2014. Working enthusiasm: the number of departments participating in hospitalwide training increased from 24 (45.28%) to 49 （92.45%）, the number of departments that carried out subsequent training increased from 21（39.62%）to 48（90.57%），departments that initiatively carried out selfinspection increased from 10（18.87%）to 43（81.13%）, differences were all significant (all P<0.001). Working ability: percentage of HCWs knew knowledge about hand hygiene as well as prevention and control measures for multidrug resistance bacteria increased from 49.05% (103/210)  and 37.14% (78/210) to 96.30% (208/216) and  97.22% (210/216) respectively; while in 2013, only 2 departments (3.77%) performed regular rectification on daily work, which increased to 19 departments （35.85%）after training，differences were all significant (all P<0.001). Working achievements: the qualified rate of correct hand hygiene among HCWs increased from 8.57%(18/210)  to 87.04%(188/216); implementation rate of multidrug resistance bacteria isolation increased from 50.00% to 80.08%，pathogenic detection in patients receiving therapeutic  antimicrobial agents increased from 28.85% to 51.30%, differences were all significant（all P＜0.001）. ConclusionFostering of hospital execution culture has improved the enthusiasm and work ability of HCWs to participate in HAI management, promoted the implementation of HAI management, and is a  powerful tool for the management of HAI.