ObjectiveTo study the homology and clinical distribution of tigecyclineresistant Acinetobacter baumannii (A. baumannii) in a hospital. MethodsMultidrugresistant A. baumannii (MDRAB, n=88) from specimens from  clinical departments of a hospital in 2013-2014 were collected and detected susceptibility to tigecycline; homology of tigecyclineresistant strains were detected by pulsedfield gel electrophoresis (PFGE), clinical characteristics and distribution of infected patients were analyzed. Results88 patients didn’t use tigecycline before MDRAB were isolated. Of 88 MDRAB strains, 4 (4.55%）were resistant to tigecycline, which were No. 10, 31,33, and 87 strains. PFGE results revealed that No. 31,33,and 87 strains were of the same genotype, and with high homology, which distributed in three different departments; No.31 strain was detected from general intensive care unit (ICU), No.33 strain was detected from emergency  ICU, although strains were  detected from different departments, patients were transferred before strains were isolated, and were admitted to departments of gastrointestinal surgery and emergency ICU during the same period; No.87 strain was detected from neurosurgical ICU and patient had never been transferred, the detection time was 7-8 months later than No. 31 and 33 strains. No.10 strain was isolated from emergency ICU, patient was not transferred.ConclusionOf MDRAB isolated in this hospital,  tigecyclineresistant strains are low, most strains are homologous, cross infection may be exists in different departments.