ObjectiveTo summarize the nursing experience in the prevention and control of Ebola virus disease(EVD), and provide reference for nursing practice on infection control management of further epidemic situation. MethodsPersonal experience in 53day fighting against EVD was retrospectively analyzed, nursing experience in the prevention and control of EVD was summarized by author who was one of 5th  China (Hunan) medical team members and head of nursing. ResultsThe whole medical team consisted of 40 health care workers(HCWs), none of them infected with Ebola virus; there was no cross infection among 105 HCWs of Sierra Leone, either, 100% of Ebola patients were cured. ConclusionBuilding up team work, strictly training and implementing standard working process,  focusing on infection control and management of treatment center are important factors that ensure zero infection among nursing staff during the process of diagnosis and treatment.