ObjectiveTo understand current status of occupational protection awareness and behavior among different professional medical interns, and provide basis for strengthening the education about occupational protection among medical interns .MethodsQuestionnaire survey was used to survey medical interns at the end of their clinical internship. ResultsA total of 385 cases of occupational exposure occurred during internship period, occupational exposure rate was 42.31% (385/910)，occupational exposure mainly occurred in nursing interns (n=190). 358（39.34%）medical interns understood selfprotective antibody (hepatitis B surface antibody); difference in occupational exposure rates and awareness of selfprotective antibody among different professional medical interns was statistically significant (all P<0.05); 222 （57.66%）medical interns sustained occupational exposure more than once, 45.45% of occupational exposure occurred during the process of diagnostic and therapeutic procedure, exposure mainly occurred in the wards（32.21%）. After occupational exposure, 46.75% of medical interns felt nervous, 35.06% were worried，18.18% appeared serious psychological reaction, only 14.03% sought support and help from others. ConclusionAwareness of occupational protection among medical interns is insufficient, education and awareness of occupational protection should be intensified, psychological behavioral after occupational exposure should be paid attention among medical students.