ObjectiveTo understand the distribution characteristics and antimicrobial resistance of Salmonella isolated from feces of children. MethodsSalmonella were isolated from fecal specimens of children in a hospital between January 2012 and October 2015, serotypes and susceptibility of Salmonella to commonly used antimicrobial agents were analyzed. ResultsA total of 7 047 children’s fecal specimens were detected, 459  (6.51%) children were isolated  Salmonella, the ratio of male to female was 2.4∶1, 352 (76.69%)  children were less than 3 years old; 374 strains (81.48%） were isolated from May to October, the numbers of stains isolated in July, August, and September were 72, 84, and 78 respectively. Salmonella group B and group D were the most common serotypes, accounting for 69.06% and 19.39% respectively, most were serotypes of Salmonella typhimurium (S. typhimurium) and Salmonella enteritidis (S. enteritidis). No imipenem or ertapenemresistant strains were found, susceptibility of Salmonella to piperacillin/tazobactam, cefepime, ceftazidime, aztreonam, and ceftriaxone were all>70%, susceptibility rate of S. enteritidis to cephalosporins was lower than S. typhimurium, but to compound sulfamethoxazole was higher than S. typhimurium. ConclusionSalmonella are mainly isolated from children less than 3 years old in this hospital, summer and autumn are peak seasons, the main serotypes were S. typhimurium and S. enteritidis, antimicrobial therapy should be carefully selected in combination with serotypes and antimicrobial susceptibility testing result.