ObjectiveTo investigate the efficacy of bundle care under the supervision of professionals in preventing ventilatorassociated pneumonia(VAP). MethodsPatients who received mechanical ventilation between July 2013 and June 2014 in the intensive care unit(ICU) of a hospital were selected, JulyDecember 2013 was as control group (n=150), patients received bundle care, but without special supervision； JanuaryJune 2014 was as special supervision group (n=177), patients received bundle care under the supervision of professionals, incidence of VAP, duration of mechanical ventilation, and length of ICU stay were compared between two groups. ResultsIn special supervision group, 141 patients received invasive mechanical ventilation, mechanical ventilation day were 1 937 days, 9 patients developed VAP, incidence of VAP was 4.65‰; in control group, 127 patients received invasive mechanical ventilation, mechanical ventilation day were 1 965 days, 21 patients developed VAP, incidence of VAP was 10.69‰, difference was statistically significant between two groups（χ2=5.68，P=0.042）. In special supervision group, overall compliance rate of bundle management strategies, duration of average invasive mechanical ventilation， and length of ICU stay were 94.92%,（11.11±2.57）days, and（15.11±2.88）days respectively，in control group were 48.67%,（14.67±4.35）days, and（19.33±5.81）days respectively，difference was statistically significant between two groups (all P＜0.05）. ConclusionBundle care strategies supervised by professionals can effectively prevent the occurrence of VAP, the implementation of bundle care strategies should be set up according to the clinical practice.