ObjectiveTo survey the point prevalence of healthcareassociated infection(HAI) in 13 tertiary hospitals in Dongguan, understand the occurrence of HAI in tertiary hospitals, so as to provide guidance for the prevention and control of HAI. MethodsThrough bedside investigation and medical record reviewing, HAI among inpatients in 13 tertiary hospitals in Dongguan at 0：00-24：00 of September 24, 2014 were investigated. ResultsA total of 11 344 patients were investigated, 379 patients developed 404 times of HAI, HAI rate was 3.34%, HAI case rate was 3.56%. Incidence of HAI in the intensive care unit was highest (12.82%). The main infection site was respiratory tract（n=173, 42.48%）. The usage rate of antimicrobial agents was 30.50%, most were for therapeutic use (including therapeutic+ prophylaxis) （n=2 545, 73.55%）and single use （n=2 689, 77.72%），pathogenic microorganism detection of specimens from patients receiving therapeutic antimicrobial use was 53.79%. A total of 299 strains of pathogenic orgamisms were detected from infected patients, the major were Escherichia coli (n=56, 18.73%), Pseudomonas aeruginosa (n=54, 18.06%), and Klebsiella pneumoniae (n=35, 11.71%), 92 strains of multidrugresistant organisms(MDROs) were isolated, accounting for 30.77% of total isolated pathogens. ConclusionThe basic characteristics of HAI in tertiary hospitals in this city were preliminarily investigated, identification of high risk departments, high risk population, and high risk links should be strengthened, HAI prevention and control measures should be implemented.