ObjectiveTo investigate the change in antimicrobial susceptibility of Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) isolated from clinical urine specimens, so as to provide laboratory evidence for clinical antiinfective treatment.MethodsAntimicrobial susceptibility of E. faecalis and E. faecium isolated from urine specimens from 20 tertiary hosptials in China between 2004 and 2014 were analyzed, drugresistant genes of vancomycinresistant Enterococcus（VRE）were detected with polymerase chain reaction (PCR).ResultsA total of 788 Enterococcus strains were isolated in 2004-2014, 371 strains were E. faecalis strains, 417 were E. faecium strains. Susceptibility rates of E. faecalis to ampicillin, nitrofurantoin, fosfomycin, vancomycin, and teicoplanin were all>90%, susceptibility rates to rifampin, minocycline, and erythromycin were all<20%, there was significant difference in the susceptibility rate of E. faecalis to fosfomycin betwen July 2011June 2012 and July 2009June 2010(P<0.0167). Susceptibility rates of E. faecium to vancomycin and teicoplanin were 96.9% and 97.4% respectively, susceptibility rates to nitrofurantoin, minocycline, and fosfomycin were 41.7%, 51.8%, and 78.2% respectively, susceptibility rates to ampicillin, levofloxacin, rifampicin, and erythromycin were all<10%; susceptibility rates of E. faecium to nitrofurantoin had decreased tendency in different years (any two group comparison, all P<0.0167), susceptibility rates to fosfomycin in July 2011June 2012 and July 2013June 2014 both decreased compared with July 2009June 2010(both P<0.0167），there were no significant changes in antimicrobial usceptibility rates in different years. 14 strains of VRE all carried vanA resistance gene.ConclusionE. faecalis strains isolated from urine are susceptible to ampicillin, nitrofurantoin, and fosfomycin, E. faecium are not susceptible to most antimicrobial agents; E. faecalis and E. faecium are both susceptible to vancomycin and teicoplanin, only a few strains are resistant to antimicrobial agents.