ObjectiveTo investigate the age distribution and types of human papillomavirus (HPV) cervical infection in patients in Wuhan region, so as to provide scientific basis for the prevention and treatment of HPV infection and cervical cancer. Methods9 915 exfoliated cervical cell specimens from patients in Wuhan Medical and Health Center for Women and Children between January 2015 and March 2016 were performed HPV genotyping (21 subtypes), clinical data of 1 732 HPV positive cases were statistically analyzed. ResultsHPVpositive rates in ≤25, 26～, 36～, 46～, and ≥56 age groups were 22.15%, 15.90%, 17.04%, 19.97%, and 17.57%，respectively (χ2=36.587，P＜0.01)，HPVpositive rates in ≤25 and 46～ age groups were both higher than other age groups. There were significant differences in single infection and multiple infection rates among different age groups respectively（χ2=14.39, 36.51，respectively, both P＜0.05），single infection rate was highest in 46～ and ≤25 age groups (15.41% and 15.24% respectively)；multiple infection rates was highest in ≤25 and ≥56 age groups (6.90% and 5.86% respectively). The percentage of single infection and multiple infection were 75.58% and 24.42% respectively, the major single infection type was HPV highrisk subtype （84.34%），the major multiple infection types were highrisk and highrisk compound subtype as well as highrisk and lowrisk compound subtype, accounting for 60.52% and 38.77% respectively. The main single infection types were HPV highrisk subtypes 33, 68, 31 and 16, as well as and lowrisk subtype 11, the ratios of single infection to multiple infection were 3.13, 2.03, 1.71, 1.67 and 2.00 respectively. ConclusionCervical infection rates in women in Wuhan region is high in ≤25 and 46～ age groups, there are differences in the distribution of different HPV subtypes of single infection and multiple infection.