ObjectiveTo carry out patients’ participation in promoting health care workers(HCWs) to improve hand hygiene (HH) compliance in the outpatient transfusion room, obtain real data of HH, and further provide basis for performing the activity in the whole hospital. MethodsHH compliance of nurses in outpatient transfusion room during four stages was observed by using the healthcareassociated infection control toolkit mobile phone APP. Intervention was not implemented at the first stage, HH promotion was implemented at the second stage, simulation of patients participated in promoting HCWs to improve HH compliance was performed at the third stage, and patients actually participated in promoting HCWs to improve HH compliance at the fourth stage. A questionnaire about patients’ willingness to participate in the promotion of HCWs to improve HH compliance was developed and filled out by patients and nurses. ResultsHH compliance rates of nurses in transfusion room at four stages were 21.43%, 44.27%, 61.48%, and 82.88% respectively, differences was significant（χ2=110.35，P＜0.01）; HH correct rates at four stages were 44.44%, 74.14%, 81.33%, and 81.82% respectively, differences was significant（χ2=18.50，P＜0.01）. ConclusionPatients participating in promotion of HH can significantly improve HH compliance of HCWs, which is simple and effective, and worth to be popularized.