ObjectiveTo investigate the current status of multidrugresistant organism (MDRO) infection in the general surgery department of a tertiary firstclass hospital, and understand the efficacy of comprehensive intervention measures. MethodsTargeted monitoring on MDROs was implemented among patients who were hospitalized in a general surgery department for>48 hours between March 2015 and October 2016, comprehensive intervention measures were taken since January 2016. MDRO infection before and after intervention was compared. ResultsBetween March 2015 and October 2016, a total of 6 406 hospitalized patients were monitored, 155(2.42%) developed MDRO infection. The most common infection sites were abdominal cavity(hydrops, bile) (n=76, 49.03%) and surgical site (n=43, 27.74%). MDRO infection rate dropped from 2.79% (99/3 549) before intervention (MarchDecember, 2015) to 1.96% (56/2 857) after intervention (JanuaryOctober, 2016) (χ2=4.612, P=0.032). There was no difference in MDROs between before and after intervention, the major were both Escherichia coli. ConclusionComprehensive intervention measures can reduce the incidence of MDRO infection in the general surgery department of this hospital.