ObjectiveTo explore the clinical characteristics and risk factors of healthcareassociated infection(HAI) in patients with severe viral hepatitis, so as to provide basis for clinical prevention and treatment of HAI. MethodsClinical data of 193 patients with severe viral hepatitis and admitted to a hospital from January 2012 to November 2015 were analyzed retrospectively, clinical characteristics and risk factors for HAI in patients were evaluated, all data were statistically analyzed. ResultsAmong 193 patients, 75 developed 112 times of HAI, incidence and case incidence of HAI were 38.86% and 58.03% respectively. The main infection sites were abdominal cavity, lower respiratory tract, and gastrointestinal tract, infection rates were 33.93%, 25.00% and 16.96% respectively. A total of 58 strains of pathogens were isolated, including fungi, gramnegative bacteria, and grampositive bacteria, accounting for 53.45%, 36.21%, and 10.34% respectively. Mulvariate logistic regression analysis showed that underlying diseases, use of glucocorticoid, invasive procedure, and white blood cell count＜4.0×109/L were independent risk factors for HAI in patients with severe viral hepatitis (P<0.05). ConclusionIncidence of HAI in patients with severe viral hepatitis is high, risk factors are multiple. It is necessary to take targeted prevention measures during the treatment, so as to reduce the incidence of HAI.