ObjectiveTo explore the practice and efficacy of multidisciplinary collaborative management in the prevention and control of multidrugresistant organism (MDRO) healthcareassociated infection(HAI). MethodsMDROs isolated from clinical specimens and MDRO infection in hospitalized patients in April 2012March 2013 (before intervention) and April 2013March 2014 (after intervention) were analyzed retrospectively. Since April 2013, multidisciplinary collaborative management has been implemented, isolation of MDROs and MDRO HAI in hospitalized patients before and after intervention were compared and analyzed. Results798 and 833 MDRO strains were isolated before and after the intervention respectively, isolation rate of MDROs after intervention was lower than that before intervention (25.71% vs 31.89%, P＜0.001）. After the implementation of multidisciplinary collaborative management, incidence of MDRO HAI decreased from 0.94% before intervention to 0.63%； hand hygiene compliance rate of health care workers(HCWs) increased from 36.44% before intervention to 53.51%; compliance rate of contact isolation increased from 65.29% before intervention to 90.88%; rational usage rate of antimicrobial agents increased from 64.93% before intervention to 72.53%; specimen detection rate in patients with therapeutic antimicrobial use increased from 41.36% before intervention to 58.72%, differences were all statistically significant (all P<0.001). ConclusionThe practice of multidisciplinary collaborative management can effectively implement the prevention and control measures of MDRO HAI, effectively reduce the occurrence of MDRO HAI