ObjectiveTo understand the clinical distribution and antimicrobial resistance of Enterococcus spp. isolated from blood culture. MethodsEnterococcus spp. isolated from blood culture specimens of hospitalized patients between January 2012 and December 2016 was analyzed, antimicrobial susceptibility testing results were analyzed. ResultsA total of 139 strains of Enterococcus spp. were isolated, including 78 strains of Enterococcus faecium (E. faecium), 60 Enterococcus faecalis (E. faecalis), and 1 Enterococcus avium(E. avium), accounting for 56.11%, 43.17%, and 0.72% respectively; 56.83% of patients were >60 years and 17.27% were ≤28 days. The main department sources of strains were intensive care unit and neonatal department, accounting for 34.53% and 18.70% respectively. There was only 1 strain of E. avium, which was isolated from neonatal department. Resistance rates of E. faecium to penicillin, ampicillin, levofloxacin, ciprofloxacin, moxifloxacin, and nitrofurantoin were all higher than E. faecalis（all P＜0.05）, while resistance rate to tetracycline was lower than E. faecalis（P=0.001）. Resistance rates of E. faecium to vancomycin and linezolid were 2.56% and 6.41% respectively; resistance rates of E. faecalis to vancomycin and linezolid were 0 and 3.33% respectively. ConclusionE. faecium and E. faecalis are the main Enterococcus spp. isolated from blood culture, most are isolated from the elderly patients and newborns. Resistance rates of E. faecium to most antimicrobial agents are higher than E. faecalis, resistance rates of E. faecium and E. faecalis to linezolid are both higher than resistance rates to vancomycin, key monitoring needs to be continued.