ObjectiveTo investigate the clinical distribution and antimicrobial resistance of carbapenemresistant Enterobacteriaceae (CRE) in Dongguan. MethodsCRE isolated from hospitalized patients in 22 secondary and above medical institutions which participated in bacterial monitoring in Dongguan between January 2015 and June 2016 were retrospectively analyzed, antimicrobial resistance was analyzed by WHONET 5.6 software. ResultsA total of 71 CRE isolates were detected, with a isolation rate of 0.34% (71/20 713). 53 strains(74.65%) of CRE were isolated from patients aged 15-60 years old; 46(64.79%) were from male patients；CRE were mainly isolated from patients in intensive care unit(36 strains，50.70%）；the main specimen was sputum(34 strains, 47.89%），followed by urine （11strians，15.49%）and wound secretion（6 strains，8.45%）；the main infection type was healthcare associated infection (64 strains, 90.14%); CRE were mainly distributed in tertiary hospitals（56 strains，78.87%）, the isolation rate of CRE in tertiary and secondary hospitals were 0.41%（56/13 677）and 0.21%（15/7 036） respectively. 71 strains of CRE were all resistant to imipenem, resistance rate to meropenem was 81.12%， only amikacin and tobramycin had drug resistance rates of <40%( 21.38% and 38.79% respectively）, resistance rate to trimethoprim/sulfamethoxazole was 48.23%，while resistance rates to fluoroquinolones, thirdgeneration cephalosporins, and enzyme inhibitors were all>60. ConclusionThe isolation rate of CRE in Dongguan is lower than that of the whole nation and the other provinces, effective prevention and control measures should be taken according to the key population and departments that isolated CRE, antimicrobial use should be rational.