ObjectiveTo explore the application efficacy of quality control circle（QCC） in hand hygiene(HH) management of laboratory staff.MethodsLaboratory staff in a hospital in Neijiang from January to December 2016 were selected as the studied objects, QCC group of HH management of laboratory staff was established, causes of correct rate of HH in laboratory staff were analyzed, corresponding countermeasures were formulated and implemented; data before and after performing QCC activity were compared and analyzed, efficacy of activities was assessed.ResultsAfter implementing QCC activity, correct rate of HH in laboratory staff increased from 29.73% to 91.04%, difference was significant(P<0.001). At the same time, circle members’ ability in QCC skill, responsibility, sense of honor, selfconfidence, cohesiveness were all promoted with varying degrees.ConclusionThe implementation of QCC activities can not only improve the correct rate of HH of laboratory staff, but also enhance team confidence and cohesiveness, it is worthy of further clinical application.