ObjectiveTo investigate drug resistance genes and epidemic characteristics of βlactamase carried by carbapenemresistant Acinetobacter baumannii(CRAB) in the respiratory intensive care unit(RICU) in a hospital.MethodsClinically isolated CRAB from RICU patients in OctoberDecember 2015 were collected. Five drug resistance genes (KPC2, IMP, VIM, NDM1, OXA23) were specifically amplified by polymerase chain reaction (PCR), amplified products were performed agarose gel electrophoresis and sequencing analysis, the homology was analyzed with pulsedfield gel electrophoresis (PFGE). ResultsA total of 22 CRAB strains were isolated in OctoberDecember 2015, 19 (86.36%) of which were isolated from sputum. The resistance rate of 22 CRAB strains to compound sulfamethoxazole was 59.09%, resistance rate to minocycline was 9.09%, all were sensitive to polymyxin B, resistance rates to other antimicrobial agents were more than 80%. Three kinds of resistance genes KPC2, IMP and NDM1 were not found by PCR amplification, positive rates of VIM and OXA23 were both 100%. PFGE homology analysis revealed that 22 strains were divided into 13 different types, each type contained 15 strains, 9 types（69.23%） contained only 1 strain respectively, the other 4 types (30.77%) contained 25 strains. A5, A7, and A8; A9, A11, A14, A19 and A22; A4, A10 and A12; A16 and A18 were of the same type respectively. ConclusionThe main types of βlactamaseresistant genes of CRAB in RICU are VIM and OXA23. Homology analysis shows a small parts are of the same clone strains, which reveals epidemic of a small scale.